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Registros recuperados: 13 | |
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Leal,C.L.V.; Méo,S.C.; Garcia,J.M.. |
O objetivo deste trabalho foi avaliar as taxas de ativação e de clivagem de oócitos bovinos tratados com estrôncio (10mm de SrCl2), após maturação in vitro por 27-28 horas. No experimento 1, os tratamentos foram: S4 - ativação pelo estrôncio por 4 horas; S12 - ativação pelo estrôncio por 12 horas; S30 - ativação pelo estrôncio por 30 horas; e P - ativação por pulso elétrico (3 pulsos de 1,0kv/cm). No experimento 2 os tratamentos foram: PS4 - ativação combinada pelo pulso elétrico e pelo estrôncio por 4 horas; S4P - ativação pelo estrôncio por 4 horas e pelo pulso elétrico; e PS30 - ativação pelo pulso elétrico e pelo estrôncio por 30 horas. No experimento 1, todos os tratamentos apresentaram taxas similares de ativação (83-90%; P>0,05). Para clivagem, P... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cattle; Activation; Parthenogenesis; Oocyte; Strontium; Electric pulse. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352003000300020 |
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Feng,D.Q.; Zhou,Y.; Ling,B.; Gao,T.; Shi,Y.Y.; Wei,H.M.; Tian,Z.G.. |
Mesenchymal stem cells (MSCs) have been reported to secrete a variety of cytokines and growth factors acting as trophic suppliers, but little is known regarding the effects of conditioned medium (CM) of MSCs isolated from femurs and tibias of mouse on the artificial activation of mouse oocytes and on the developmental competence of the parthenotes. In the current study, we investigated the effect of CM on the events of mouse oocyte activation, namely oscillations of cytosolic calcium concentration ([Ca²+]i), meiosis resumption, pronucleus formation, and parthenogenetic development. The surface markers of MSCs were identified with a fluorescence-activated cell sorter. The dynamic changes of the spindle and formation of pronuclei were examined by... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mesenchymal stem cells; Oocyte activation; Parthenogenesis; Calcium; Cytochalasin B. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600006 |
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Cosson, J; Gayral, P. |
The research has shown that interspecific and intergeneric hybridization is possible in the Laminariales with variable rates according to the species used; these rates were determined precisely only following preliminary study where the rate of parthenogenetic development was evaluated for each particular species. Besides, the results obtained concerning the ecophysiology of the Laminariales and especially the effect of temperature and light on the fertility of the gametophytes, prompt to undertake experimental cultures of the hybrids in order to obtain algae with qualities superior to those of the parents, particularly regarding alginic acid content and growth. The experiments carried out in 1983 concern together the rearing ashore of isolated... |
Tipo: Text |
Palavras-chave: Laminaria; Algae; Algal culture; Parthenogenesis; Hybrid culture; Seaweed culture. |
Ano: 1983 |
URL: http://archimer.ifremer.fr/doc/1983/acte-1173.pdf |
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Perecin,F.; Méo,S.C.; Leal,C.L.V.; Garcia,J.M.. |
The efficiency of bohemine and roscovitine in combination with ionomycin on parthenogenetic activation and initial embryonic development of bovine oocytes was studied. Two experiments were performed: in the first, different concentrations (0, 50, 75 or 100µM) and different exposure periods (2, 4 or 6 hours) to bohemine or roscovitine were tested for activation rates of in vitro matured (IVM) bovine oocytes, which were pre-exposed to ionomycin. The best treatments, 75µM bohemine and 50µM roscovitine, both for 6h, were used in the second experiment, in which IVM bovine oocytes were exposed to ionomycin, followed or not by bohemine or roscovitine treatment, and evaluated for nuclear status, activation rate and blastocyst development were assessed. The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; Artificial activation; Bohemine; Roscovitine; Parthenogenesis. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000200002 |
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LOURENÇO,W.R.; CUELLAR,O.. |
Recently public health problems resulting from scorpion stings have shown an alarming increase in various tropical and subtropical countries such as Brazil, Mexico, Tunisia and Morocco. In some regions of Brazil, particularly in the States of Minas Gerais, São Paulo, Bahia, Goiás and the Federal District, more than 6000 scorpion stings with over 100 deaths were reported during a three-year period. In this paper, we attempt to demostrate the effects of human activities on the environment and their consequences on the distribution of dangerous species of scorpions. This topic is discussed in relation to the evolutionary ecology, life history strategies and asexual reproduction of scorpions. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Scorpionism; Scorpion; Life history strategy; Parthenogenesis; Brazil. |
Ano: 1995 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301995000200002 |
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Registros recuperados: 13 | |
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